Peterson M J, Morris J F
Medical College of Wisconsin, Kelly Weil Laboratory of Pediatric Molecular Oncology, Departments of Pediatrics and Biochemistry, MFRC 6008, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA.
Gene. 2000 Aug 22;254(1-2):105-18. doi: 10.1016/s0378-1119(00)00281-x.
The myeloid zinc finger gene 1 (MZF1) encodes a C(2)H(2) zinc finger transcription factor that regulates granulopoiesis and may have a regulatory role in cellular proliferation and oncogenesis. The MZF1 gene has been previously reported to be 3kb and without introns. However, at least three transcripts of approximately 3, 7.5, and 9kb are detected by MZF1-specific probes in northern blot analysis and the identity of the transcripts has not been addressed. We screened a K562 cDNA library and identified novel transcripts, MZF1B and MZF1C. The 2.9kb MZF1B cDNA encodes a putative 734 aa protein and MZF1C maintains an identical open reading frame with 320 nucleotides deleted in the 5'-untranslated region. The MZF1B/1C protein contains all but the first eight amino acids of MZF1. Thus, MZF protein isoforms share 100 aa, as well as the bipartite 13 zinc finger DNA binding domain. In addition, MZF1B/1C encodes a unique 257 aa MZF1B/C amino terminus containing a SCAN box, or leucine-rich domain, which has recently been demonstrated to facilitate protein interactions. Sequence analysis reveals that the MZF gene contains six exons and spans 11kb and may be the most telomeric gene on chromosome 19q13. Exons 1-6 produce MZF1B/C cDNA, whereas MZF1 cDNA initiates within intron 5 and continues through exon 6. The 7.5 and 9kb transcripts are incompletely processed and contain intron sequences. These studies are the first description of the complete human MZF gene and of the composition of the multiple transcripts that are detected by northern blot analysis.
髓系锌指基因1（MZF1）编码一种C(2)H(2)锌指转录因子，该因子调节粒细胞生成，并且可能在细胞增殖和肿瘤发生中起调控作用。先前报道MZF1基因长度为3kb且无内含子。然而，在Northern印迹分析中，用MZF1特异性探针检测到至少三种大小约为3、7.5和9kb的转录本，且这些转录本的身份尚未明确。我们筛选了一个K562 cDNA文库并鉴定出了新的转录本MZF1B和MZF1C。2.9kb的MZF1B cDNA编码一个推定的734个氨基酸的蛋白质，而MZF1C与MZF1B保持相同的开放阅读框，但在5'-非翻译区缺失了320个核苷酸。MZF1B/1C蛋白包含MZF1除前八个氨基酸外的所有氨基酸。因此，MZF蛋白异构体共享100个氨基酸，以及双分型的13个锌指DNA结合结构域。此外，MZF1B/1C编码一个独特的257个氨基酸的MZF1B/C氨基末端，其中包含一个SCAN框或富含亮氨酸结构域，最近已证明该结构域有助于蛋白质相互作用。序列分析表明，MZF基因包含六个外显子，跨度为11kb，可能是19q13染色体上最末端的基因。外显子1-6产生MZF1B/C cDNA，而MZF1 cDNA起始于内含子5内并延续至外显子6。7.5kb和9kb的转录本加工不完全，包含内含子序列。这些研究首次描述了完整的人类MZF基因以及通过Northern印迹分析检测到的多种转录本的组成。
