Gryczyński M, Kobos J, Niewiadomska H, Pietruszewska W
Katedra i Klinika Otolaryngologii AM w Lodzi.
Otolaryngol Pol. 2000;54 Suppl 31:191-5.
Kinetics (growth fraction of tumour cell populations), death process of cancer cells (apoptosis and necrosis) and neovascularisation in tumour (angiogenesis) have influence on the growth of cancer. Sixty patients with laryngeal cancer treated in ENT Department of Medical Academy of Lodz were analysed. Proliferation activity of cancer cells was examined by means of selection appropriate antigen (Ki-67) characteristic for cell cycle utilising immunohistochemical techniques carried out on laryngeal cancer paraffin samples. Expression of selected protein connected with apoptosis (p-53) and intensity of angiogenesis were examine using selected antibody (anti-CD34) aimed against epithelial antigens. Above-mentioned markers were correlated with: stage of cancer progression, recurrences and metastasis of laryngeal cancer and follow-up of the patients. The morphological properties were examined as well. The researches on apoptosis, angiogenesis and proliferation of cancer cells can be used as prognostic factors for the patients with laryngeal cancer.
肿瘤细胞群体的动力学（生长分数）、癌细胞的死亡过程（凋亡和坏死）以及肿瘤中的新血管形成（血管生成）都会影响癌症的生长。对罗兹医学院耳鼻喉科治疗的60例喉癌患者进行了分析。利用在喉癌石蜡样本上进行的免疫组织化学技术，通过选择适合细胞周期的抗原（Ki-67）来检测癌细胞的增殖活性。使用针对上皮抗原的选定抗体（抗CD34）检测与凋亡相关的选定蛋白质（p-53）的表达以及血管生成强度。上述标志物与喉癌的癌症进展阶段、复发和转移以及患者随访情况相关。同时也对形态学特性进行了检查。对癌细胞凋亡、血管生成和增殖的研究可作为喉癌患者的预后因素。
