Nagaraja P, Sunitha K R, Silwadi M F
Department of Studies in Chemistry, Mysore University, Manasagangotri, India.
J Pharm Biomed Anal. 2000 Sep;23(4):617-22. doi: 10.1016/s0731-7085(00)00319-8.
A sensitive and simple spectrophotometric method for the determinations of reduction product of flutamide (FLA) is described. The method is based on the interaction of diazotized flutamide reduction product with N-(1-naphthyl) ethylenediamine dihydrochloride (NEDA) in neutral or resorcinol (RSL) in alkaline medium. Absorbance of the resulting chromophores is measured at 525 or 480 nm, respectively, and is stable for at least 7 days. The two coupling reagents are applied successfully for the determination of FLA in tablets. The common excipients used as additives in pharmaceutical preparations do not interfere with the determination. Results from the analysis of pure FLA and its commercial tablets by the proposed methods agree well with the reported method.
描述了一种灵敏且简便的分光光度法用于测定氟他胺（FLA）的还原产物。该方法基于重氮化的氟他胺还原产物与中性介质中的N-（1-萘基）乙二胺二盐酸盐（NEDA）或碱性介质中的间苯二酚（RSL）之间的相互作用。分别在525或480nm处测量所得发色团的吸光度，且至少7天稳定。这两种偶联试剂成功用于片剂中FLA的测定。药物制剂中用作添加剂的常见辅料不干扰测定。用所提出的方法对纯FLA及其市售片剂进行分析的结果与报道的方法吻合良好。
