Archer D B
J Gen Microbiol. 1975 Jun;88(2):329-38. doi: 10.1099/00221287-88-2-329.
Mycoplasma mycoides subsp. capri was grown in different media. The amounts of the lipids in these media were varied, resulting in altered lipid compositions of the cells. Lowering the amounts of cholesterol in the media resulted in less cholesterol incorporation into the cell lipid, with a concomitant decrease in the amount of phospholipid. The changes in the phospholipid/cholesterol ratio in the mycoplasmas were very small compared with the large changes in the amounts of cholesterol which occurred when the cholesterol content in the medium was altered. Changes in the amounts of glycolipid and glyceride in the cell lipid also resulted from alterations of the cholesterol concentration in the media. Under these conditions cell with reduced cholesterol contents were more sensitive to lysis by digitonin. No changes were observed in the polyacrylamide gel electrophoretic pattern of cellular or membrane proteins when the cholesterol was replaced by other sterols.
山羊支原体丝状亚种在不同培养基中培养。这些培养基中脂质的含量各不相同，导致细胞的脂质组成发生改变。降低培养基中胆固醇的含量会使细胞脂质中胆固醇的掺入量减少，同时磷脂的量也会减少。与培养基中胆固醇含量改变时胆固醇量的大幅变化相比，支原体中磷脂/胆固醇比值的变化非常小。培养基中胆固醇浓度的改变也导致细胞脂质中糖脂和甘油酯含量的变化。在这些条件下，胆固醇含量降低的细胞对洋地黄皂苷的裂解更敏感。当胆固醇被其他固醇替代时，细胞或膜蛋白的聚丙烯酰胺凝胶电泳图谱未观察到变化。
