Hsu A C, Dawson M J
Biomedical Magnetic Resonance Laboratory, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
Magn Reson Med. 2000 Sep;44(3):418-26. doi: 10.1002/1522-2594(200009)44:3<418::aid-mrm12>3.0.co;2-g.
As the end product of anaerobic metabolism and a source of H(+), lactic acid is important in metabolism and pH regulation. Several methods have been introduced to calculate changes in the lactate anion (Lac(-)) concentration in exercising skeletal muscle from information derived from the (31)P spectrum. Alternatively, Lac-may be observed directly with (1)H MRS. Both (1)H and (31)P spectroscopy have potential problems, which could prevent accurate determination of [Lac(-)]. It is demonstrated that quantitatively accurate (1)H MRS measurements of changes in [Lac(-)] due to exercise are possible in isolated muscle. In general, calculation by (31)P MRS overestimates Lac-production. An analysis is presented of possible sources of errors in the (1)H and (31)P MRS methods.
作为无氧代谢的终产物和H⁺的来源，乳酸在代谢和pH调节中很重要。已经引入了几种方法，根据从³¹P谱获得的信息来计算运动骨骼肌中乳酸阴离子（Lac⁻）浓度的变化。或者，可以用¹H磁共振波谱（¹H MRS）直接观察Lac⁻。¹H和³¹P波谱都存在潜在问题，这可能会妨碍对[Lac⁻]的准确测定。结果表明，在离体肌肉中，通过¹H MRS对运动引起的[Lac⁻]变化进行定量准确测量是可行的。一般来说，用³¹P MRS计算会高估乳酸的产生量。本文分析了¹H和³¹P MRS方法中可能的误差来源。
