Cava F, González C, Pascual M J, Navajo J A, González-Buitrago J M
Servicio de Bioquímica, Hospital Universitario, Salamanca, Spain.
Cytokine. 2000 Sep;12(9):1423-5. doi: 10.1006/cyto.2000.0714.
The biological variation of interleukin 6 (IL-6) and soluble interleukin 2 receptor (sIL2R), measured by automated enzyme immunoassay, in fifteen subjects studied at regular monthly intervals over a period of 6 consecutive months was measured. The mean and standard deviation (SD), within-subject CV, between-subject CV, individuality index (II) and reliability coefficient (R) were as follow: for sIL2R 571 (231) U/ml, 5.84%, 38.81%, 0.21 and 0.93; and for IL-6 1.43 (0.9) pg/ml, 48.48%, 39.38%, 1.44, and 0.37. The data indicate a relatively high between-subject CV, quite similar in both cases, and a within-subject CV much higher for IL-6 than for sIL2R. Thus, reference values can be used for diagnosis for IL6 (high II), while not for sIL2R (low II). However, the low R for IL-6 implies that more than one measurement are needed. sIL2R has a very high R and a relatively small critical differences, a circumstance appropriate for follow-up.
通过自动酶免疫测定法，对15名受试者连续6个月每月定期进行检测，测量白细胞介素6（IL-6）和可溶性白细胞介素2受体（sIL2R）的生物学变异。sIL2R的均值和标准差（SD）、受试者内CV、受试者间CV、个体性指数（II）和可靠性系数（R）如下：571（231）U/ml、5.84%、38.81%、0.21和0.93；IL-6的分别为1.43（0.9）pg/ml、48.48%、39.38%、1.44和0.37。数据表明受试者间CV相对较高，两种情况相当相似，且IL-6的受试者内CV远高于sIL2R。因此，参考值可用于IL-6的诊断（个体性指数高），而不适用于sIL2R（个体性指数低）。然而，IL-6的可靠性系数低意味着需要进行不止一次测量。sIL2R的可靠性系数非常高，临界差异相对较小，适合用于随访。
