Ross R, Klebanoff S J
J Cell Biol. 1967 Jan;32(1):155-67. doi: 10.1083/jcb.32.1.155.
The fine structure of the estrogen-primed uterus was examined in two series of rats, with emphasis upon the alterations in smooth muscle cells and fibroblasts. The first series of animals were mature animals that were sacrificed at diestrus or estrus. The second series consisted of prepubertal rats (57-70 g) that received subcutaneous injections of estradiol-17 beta in 20% alcohol. Four groups of animals received the hormone twice daily for 3 days for a total dose of 0.06, 0.6, 6.0, or 60.0 microg, respectively. An estrogenic response was observed in all groups as indicated by an increase in uterine weight. Control groups consisted of either untreated animals or animals receiving 20% alcohol. All animals were sacrificed on the 4th day. The fibroblasts and smooth muscle cells in the controls were similar to their counterparts in the mature animal in diestrus. They were small, contained relatively little rough endoplasmic reticulum, and the connective tissue cells appeared like fibrocytes. All of the estrogen-treated animals were similar in appearance and were comparable to their counterparts in the mature animal in estrus. Both the smooth muscle cells and the fibroblasts were increased in size, demonstrated a marked enlargement and dilation of ergastoplasmic cisternae, and contained increased numbers of attached and free cytoplasmic ribosomes. The presence of an extensive rough endoplasmic reticulum in the smooth muscle cells of the stimulated uterus is in marked contrast to the appearance of these cells in other tissues. These observations correlate with previous biochemical studies by other workers, in which estrogens have been shown to promote the synthesis of uterine RNA, collagen, and noncollagenous protein, and suggest that smooth muscle cells may participate in the synthesis of connective tissue proteins.
在两组大鼠中研究了雌激素预处理子宫的精细结构，重点关注平滑肌细胞和成纤维细胞的变化。第一组动物为成熟动物，在间情期或发情期处死。第二组由青春期前大鼠（57 - 70克）组成，它们皮下注射溶于20%乙醇的17β - 雌二醇。四组动物每天接受两次激素注射，共3天，总剂量分别为0.06、0.6、6.0或60.0微克。如子宫重量增加所示，所有组均观察到雌激素反应。对照组由未处理的动物或接受20%乙醇的动物组成。所有动物在第4天处死。对照组中的成纤维细胞和平滑肌细胞与处于间情期的成熟动物中的对应细胞相似。它们体积小，粗面内质网相对较少，结缔组织细胞看起来像纤维细胞。所有接受雌激素处理的动物外观相似，与处于发情期的成熟动物中的对应细胞相当。平滑肌细胞和成纤维细胞均增大，内质网池明显扩大和扩张，附着的和游离的细胞质核糖体数量增加。受刺激子宫的平滑肌细胞中广泛存在粗面内质网，这与这些细胞在其他组织中的外观形成明显对比。这些观察结果与其他研究人员先前的生化研究相关，在这些研究中已表明雌激素可促进子宫RNA、胶原蛋白和非胶原蛋白的合成，并表明平滑肌细胞可能参与结缔组织蛋白的合成。
