Ross R, Klebanoff S J
J Cell Biol. 1971 Jul;50(1):159-71. doi: 10.1083/jcb.50.1.159.
These studies have examined the ability of smooth muscle cells from developing aorta of the prepubertal rat to utilize amino acids in the synthesis and secretion of connective tissue proteins. Prepubertal rats, previously given either an alcohol carrier or estradiol-17-beta, were each given an intravenous injection of proline-(3)H. The animals were sacrificed after 15 and 30 min, and 4 hr. Light and electron microscope radioautographs of the aortic smooth muscle and of the myometrial cells demonstrated that the aortic cells, in both groups of animals, and the myometrial cells, in the estrogen-stimulated animals, took up the proline and rapidly secreted it in both collagen and elastic fibers within 4 hr. In contrast, the myometrial cells of the nonstimulated animal took up relatively small amounts of proline and retained most of the amino acid within the cells. Electron microscope radioautographs demonstrated that the organelles involved in this activity were the rough endoplasmic reticulum and Golgi complex together with peripheral elements, presumed to be small vesicles. These studies have demonstrated that the smooth muscle cells of the developing aorta and of the estrogen-stimulated myometrium have a capacity to synthesize and secrete proteins associated with the extracellular connective tissue matrix.
这些研究检测了青春期前大鼠发育中的主动脉平滑肌细胞在合成和分泌结缔组织蛋白过程中利用氨基酸的能力。给青春期前大鼠分别预先给予酒精载体或雌二醇-17-β，然后每只大鼠静脉注射脯氨酸-³H。在15分钟、30分钟和4小时后处死动物。主动脉平滑肌和子宫肌层细胞的光镜和电镜放射自显影片显示，在两组动物中，主动脉细胞以及在雌激素刺激的动物中的子宫肌层细胞，摄取了脯氨酸，并在4小时内在胶原纤维和弹性纤维中迅速分泌。相比之下，未受刺激动物的子宫肌层细胞摄取的脯氨酸相对较少，且大部分氨基酸保留在细胞内。电镜放射自显影片显示，参与此活动的细胞器是粗面内质网、高尔基体复合体以及推测为小泡的周边成分。这些研究表明，发育中的主动脉平滑肌细胞和雌激素刺激的子宫肌层细胞有能力合成和分泌与细胞外结缔组织基质相关的蛋白质。
