Liu T J, Xu W L, Sun W B, Zhang Y Z
State Key Laboratory of Microbial Technology, Shandong University, Jinan.
Sheng Wu Gong Cheng Xue Bao. 2000 Mar;16(2):229-31.
Effects of several parameters relating to dissolved oxygen(DO) on mutanolysin fermentation were studied. The experiment using shake flasks shows that the medium volume and shaker agitation speed affect the production of mutanolysin. At the same time, the agitation rate together with aeation rate has effects on DO in fermentor. Mutanolysin fermentation was affected by DO greatly. Oxygen is a key restricted factor in mutanolysin fermentation. It affects the metablism and physiological action of Streptomyces globisporus S186. Whatever the DO is excessive high or low, it won't benefit the mutanolysin production. If DO is super, S. globisporus S186 will grow luxuriantly but do not produce mutanolysin, while if DO is lower, the S. globisporus S186 won't grow well even not to produce mutanolysin. During the course of fermentation, the DO changed regularly. It is similar to many antibiotic fermentation and some amino acid fermentation. As S. globisporus S186 grow in exponential phase, DO begin to decrease rapidly from 6 h and get to the lowest point at 40 h or so. Subsequently mutanolysin starts to be produced. DO rises again from 90 h. The key technoloyg of oxygen control in the fermentation is to keep the DO at a suboptimum level. In order to get a high mutanolysin yield, during the culture in fermentor the agitation rate and aeration rate should be kept at 200 r/min and 1:0.8(V:V) respectively.
研究了几个与溶解氧（DO）相关的参数对变溶菌素发酵的影响。使用摇瓶的实验表明，培养基体积和摇床搅拌速度会影响变溶菌素的产生。同时，搅拌速率与通气速率对发酵罐中的溶解氧有影响。变溶菌素发酵受溶解氧的影响很大。氧气是变溶菌素发酵中的关键限制因素。它影响球形链霉菌S186的代谢和生理作用。无论溶解氧过高还是过低，都不利于变溶菌素的产生。如果溶解氧过高，球形链霉菌S186会生长旺盛但不产生变溶菌素，而如果溶解氧较低，球形链霉菌S186生长不佳甚至不产生变溶菌素。在发酵过程中，溶解氧有规律地变化。这与许多抗生素发酵和一些氨基酸发酵类似。随着球形链霉菌S186在指数生长期生长，溶解氧从6小时开始迅速下降，并在40小时左右达到最低点。随后开始产生变溶菌素。溶解氧从90小时再次上升。发酵中氧气控制关键技术是将溶解氧保持在次优水平。为了获得高变溶菌素产量，在发酵罐培养期间，搅拌速率和通气速率应分别保持在200转/分钟和1:0.8（V:V）。
