Tatibouët A, Yang J, Morin C, Holman G D
Department of Biology and Biochemistry, University of Bath, UK.
Bioorg Med Chem. 2000 Jul;8(7):1825-33. doi: 10.1016/s0968-0896(00)00108-5.
We have examined the specificity and binding-site spatial requirements of the fructose transporter GLUT5. Interaction with a series of fructofuranosides and fructopyranosides suggests that both furanose and pyranose ring forms of D-fructose combine with GLUT5. The epimers of D-fructose all have low affinity for GLUT5 suggesting that the transporter requires all hydroxyls to be in the fructo-configuration. Similarly there is poor tolerance of all allyl derivatives of D-fructose except 6-O-allyl-D-fructofuranose. Therefore, the C-6 position offers the most suitable position for development of affinity probes and labels for exploring GLUT5 biochemistry.
我们研究了果糖转运体GLUT5的特异性和结合位点的空间要求。与一系列呋喃果糖苷和吡喃果糖苷的相互作用表明，D-果糖的呋喃糖和吡喃糖环形式均能与GLUT5结合。D-果糖的差向异构体对GLUT5的亲和力都很低，这表明该转运体需要所有羟基处于果糖构型。同样，除了6-O-烯丙基-D-呋喃果糖外，D-果糖的所有烯丙基衍生物的耐受性都很差。因此，C-6位为开发用于探索GLUT5生物化学的亲和探针和标记物提供了最合适的位置。
