Shin T Y, Kim D K
College of Pharmacy, Woosuk University, Chonju, Chonbuk, Korea.
Arch Pharm Res. 2000 Aug;23(4):401-6. doi: 10.1007/BF02975455.
We investigated the effect of aqueous extract of Gleditsia sinensis thorns (Leguminosae) (GSAE) on the mast cell-dependent anaphylaxis. GSAE (0.005 to 1 g/kg) dose-dependently inhibited systemic anaphylaxis induced by compound 48/80 in rats. GSAE (0.1 and 1 g/kg) also significantly inhibited local anaphylaxis activated by anti-DNP IgE. When GSAE was pretreated at the same concentrations with systemic anaphylaxis, the plasma histamine levels were reduced in a dose-dependent manner. GSAE (0.001 to 1 mg/ml) dose-dependently inhibited the histamine release from rat peritoneal mast cells (RPMC) activated by compound 48/80 or anti-DNP IgE. The level of cyclic AMP in RPMC, When CSAE (1 mg/ml) was added, transiently and significantly increased about fourfold compared with that of basal cells. Moreover, GSAE (0.01 and 0.1 mg/ml) had a significant inhibitory effect on anti-DNP IgE-induced tumor necrosis factor-alpha production from RPMC. These results suggest a possible use of GSAE in managing mast cell-dependent anaphylaxis.
我们研究了皂荚刺水提取物（豆科）（GSAE）对肥大细胞依赖性过敏反应的影响。GSAE（0.005至1克/千克）剂量依赖性地抑制了化合物48/80诱导的大鼠全身性过敏反应。GSAE（0.1和1克/千克）也显著抑制了抗DNP IgE激活的局部过敏反应。当用相同浓度的GSAE预处理全身性过敏反应时，血浆组胺水平呈剂量依赖性降低。GSAE（0.001至1毫克/毫升）剂量依赖性地抑制了化合物48/80或抗DNP IgE激活的大鼠腹膜肥大细胞（RPMC）释放组胺。当加入CSAE（1毫克/毫升）时，RPMC中的环磷酸腺苷水平与基础细胞相比瞬时且显著增加了约四倍。此外，GSAE（0.01和0.1毫克/毫升）对RPMC中抗DNP IgE诱导的肿瘤坏死因子-α产生具有显著抑制作用。这些结果表明GSAE在治疗肥大细胞依赖性过敏反应方面可能具有应用价值。
