一种用于快速检测实验性系统性念珠菌病中念珠菌抗体的放射免疫测定方法。

A radioimmunoassay method for the rapid detection of Candida antibodies in experimental systemic candidiasis.

作者信息

Huang S Y, Berry C W, Newman J T, Cooper W H, Zachariah N Y

出版信息

Mycopathologia. 1979 Mar 30;67(1):55-8. doi: 10.1007/BF00436242.

Abstract

Rabbits were employed as experimental models to evaluate a solid-phase radioimmunoassay (RIA) method for the diagnosis of systemic candidiasis. Ten rabbits were inoculated subcutaneously to mimic superficial candidiasis and were found to produce no antibodies to Candida as determined by both immunodiffusion and RIA procedures. However, 94 per cent of 18 rabbits systemically infected by intravenous injection of Candida cells were observed to produce antibody as assessed by the RIA technique. These data encourage further tests with human sera and the continued development ofthis RIA procedure as a useful tool in the early serodiagnosis of systemic candidiasis.

摘要

选用兔子作为实验模型，以评估用于诊断系统性念珠菌病的固相放射免疫测定（RIA）方法。10只兔子经皮下接种以模拟浅表念珠菌病，通过免疫扩散和RIA程序测定发现其未产生抗念珠菌抗体。然而，通过RIA技术评估发现，18只经静脉注射念珠菌细胞而受到全身感染的兔子中有94%产生了抗体。这些数据促使人们进一步用人血清进行检测，并继续开发这种RIA程序，使其成为系统性念珠菌病早期血清诊断的有用工具。