Detection of IgG candida antibodies by solid-phase radioimmunoassay and comparison with agar-gel diffusion.

Comparison of solid-phase radioimmunoassay (S-PRIA) and agar-gel diffusion for detecting antibodies to candida organisms was undertaken in patients with systemic candidiasis, candida colonization, noncandida febrile illnesses, and in healthy controls. Radioimmunoassay results were highly reproducible when the same sera were tested on three different days. Sera from all three groups of patients had higher counts per minute (CPM) by S-PRIA then sera from control subjects. Although positive precipitins and high CPM occurred frequently in patients with systemic candidiasis, both precipitins and high CPM were detected in some sera from patients with colonization without system disease.