Sabelnikov A G, Avdeeva A V
Mol Gen Genet. 1975 Jul 10;138(4):351-8. doi: 10.1007/BF00264805.
The first steps in E. coli transformation have been studied. It was found that the cells attain the ability of enhanced uptake of donor exogenous DNA upon Ca2+ treatment. At saturating DNA concentrations the cells were capable to take up irreversibly about 6-10(8) dalton of donor DNA per cell (if to assume that all the cells are competent). About 75% of this DNA was found to be attached to the cytoplasmic membrane. Employing Poisson approximation for distribution of the donor-marker DNA molecules on recipient cells it was found that the efficiency of a marker recombination and expression is about 2-10(-5). The first steps in E. coli transformation have been studied. It was found that the cells attain the ability of enhanced uptake of donor 0exogenous
已经对大肠杆菌转化的初始步骤进行了研究。发现细胞经Ca2+处理后获得了增强摄取供体外源DNA的能力。在DNA浓度饱和时，细胞能够不可逆地摄取每个细胞约6 - 10(8)道尔顿的供体DNA（如果假设所有细胞都具有感受态）。发现约75%的这种DNA附着在细胞质膜上。利用泊松近似法对受体细胞上供体标记DNA分子的分布进行分析，发现标记重组和表达的效率约为2 - 10(-5)。已经对大肠杆菌转化的初始步骤进行了研究。发现细胞获得了增强摄取供体0外源……（原文此处不完整）
