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[Quantity of exogenous linear DNA absorbed by E. coli cells treated with Ca2+ cations].

作者信息

Bukrinskiĭ M I, Sabel'nikov A G

出版信息

Biull Eksp Biol Med. 1981 May;91(5):594-6.

PMID:6266558
Abstract

The amount of linear chromosomal DNA entering Ca2+-treated Escherichia coli K 12 cells was estimated from the degree of degradation of exogenous DNA by intracellular nucleases. At least 40% of DNA adsorbed by the cells in the nucleaso-resistant form (45 MD/cell) was discovered to be as intracellular. No significant difference was found between single- and double-stranded DNA.

摘要

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