Brown K A, Kriss J A, Moser C A, Wenner W J, Offit P A
Division of Gastroenterology and Nutrition, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
J Infect Dis. 2000 Oct;182(4):1039-43. doi: 10.1086/315808. Epub 2000 Sep 5.
Rotaviruses are the most important cause of infectious diarrhea in children throughout the world. Protection is most likely mediated by small-intestinal virus-specific IgA. However, neither fecal nor serum virus-specific IgA clearly correlates with protection against challenge. The capacity of rotavirus-specific antibodies and rotavirus-specific antibody-secreting cells (ASCs) in the circulation to predict the presence of ASCs in the intestines of children was evaluated. Mononuclear cells from intestinal biopsy samples and blood from 21 children were enriched for CD38, a marker of terminally differentiated B cells, and evaluated for the presence of virus-specific and total IgA- and IgG-secreting cells, by ELISPOT assay. Serum virus-specific IgA and IgG levels were determined by ELISA. The ratio of virus-specific to total IgA-secreting cells in the blood correlated with that found in the small, but not large, intestine. In contrast, serum rotavirus-specific IgA correlated less well with the presence of virus-specific ASCs in the small intestine.
轮状病毒是全世界儿童感染性腹泻的最重要病因。保护作用很可能由小肠病毒特异性IgA介导。然而，粪便和血清中的病毒特异性IgA均与抵抗攻击的保护作用无明显关联。对循环中轮状病毒特异性抗体和轮状病毒特异性抗体分泌细胞（ASC）预测儿童肠道中ASC存在的能力进行了评估。通过ELISPOT分析，对来自21名儿童的肠道活检样本中的单核细胞和血液进行终末分化B细胞标志物CD38富集，并评估病毒特异性及总IgA和IgG分泌细胞的存在情况。通过ELISA测定血清病毒特异性IgA和IgG水平。血液中病毒特异性与总IgA分泌细胞的比例与在小肠而非大肠中发现的比例相关。相比之下，血清轮状病毒特异性IgA与小肠中病毒特异性ASC的存在相关性较差。
