Yuan Lijuan, Wen Ke, Azevedo Marli S P, Gonzalez Ana M, Zhang Wei, Saif Linda J
Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH 44691, USA.
Vaccine. 2008 Jun 19;26(26):3322-31. doi: 10.1016/j.vaccine.2008.03.085. Epub 2008 Apr 18.
We examined rotavirus-specific IFN-gamma producing CD4+, CD8+ and CD4+CD8+ T cell responses in gnotobiotic pigs infected with a virulent human rotavirus (VirHRV) or vaccinated with an attenuated (Att) HRV vaccine (AttHRV3x or AttHRV2x) or an AttHRV oral priming and 2/6-virus-like particle (VLP) intranasal boosting (AttHRV-2/6VLP) regimen. In VirHRV infected pigs, HRV-specific IFN-gamma producing T cells reside primarily in ileum. AttHRV-2/6VLP induced similar frequencies of intestinal IFN-gamma producing T cells as the VirHRV, whereas AttHRV3x or 2x vaccines were less effective. Protection rates against rotavirus diarrhea upon VirHRV challenge significantly correlated (r=0.97-1.0, p<0.005) with frequencies of intestinal IFN-gamma producing T cells, suggesting their role in protective immunity.
我们检测了无菌猪感染强毒力人轮状病毒（VirHRV）或接种减毒（Att）HRV疫苗（AttHRV3x或AttHRV2x）或AttHRV口服初免和2/6病毒样颗粒（VLP）鼻内加强免疫（AttHRV-2/6VLP）方案后，产生IFN-γ的轮状病毒特异性CD4⁺、CD8⁺和CD4⁺CD8⁺T细胞反应。在感染VirHRV的猪中，产生HRV特异性IFN-γ的T细胞主要存在于回肠。AttHRV-2/6VLP诱导产生肠道IFN-γ的T细胞频率与VirHRV相似，而AttHRV3x或2x疫苗效果较差。VirHRV攻击后针对轮状病毒腹泻的保护率与肠道产生IFN-γ的T细胞频率显著相关（r=0.97-1.0，p<0.005），表明它们在保护性免疫中的作用。
