人轮状病毒感染和疫苗诱导产生的病毒特异性肠道干扰素-γ 产生性T细胞应答与悉生猪抵抗轮状病毒腹泻的保护作用相关。
Virus-specific intestinal IFN-gamma producing T cell responses induced by human rotavirus infection and vaccines are correlated with protection against rotavirus diarrhea in gnotobiotic pigs.
作者信息
Yuan Lijuan, Wen Ke, Azevedo Marli S P, Gonzalez Ana M, Zhang Wei, Saif Linda J
机构信息
Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH 44691, USA.
出版信息
Vaccine. 2008 Jun 19;26(26):3322-31. doi: 10.1016/j.vaccine.2008.03.085. Epub 2008 Apr 18.
Abstract
We examined rotavirus-specific IFN-gamma producing CD4+, CD8+ and CD4+CD8+ T cell responses in gnotobiotic pigs infected with a virulent human rotavirus (VirHRV) or vaccinated with an attenuated (Att) HRV vaccine (AttHRV3x or AttHRV2x) or an AttHRV oral priming and 2/6-virus-like particle (VLP) intranasal boosting (AttHRV-2/6VLP) regimen. In VirHRV infected pigs, HRV-specific IFN-gamma producing T cells reside primarily in ileum. AttHRV-2/6VLP induced similar frequencies of intestinal IFN-gamma producing T cells as the VirHRV, whereas AttHRV3x or 2x vaccines were less effective. Protection rates against rotavirus diarrhea upon VirHRV challenge significantly correlated (r=0.97-1.0, p<0.005) with frequencies of intestinal IFN-gamma producing T cells, suggesting their role in protective immunity.
摘要
我们检测了无菌猪感染强毒力人轮状病毒（VirHRV）或接种减毒（Att）HRV疫苗（AttHRV3x或AttHRV2x）或AttHRV口服初免和2/6病毒样颗粒（VLP）鼻内加强免疫（AttHRV-2/6VLP）方案后，产生IFN-γ的轮状病毒特异性CD4⁺、CD8⁺和CD4⁺CD8⁺T细胞反应。在感染VirHRV的猪中，产生HRV特异性IFN-γ的T细胞主要存在于回肠。AttHRV-2/6VLP诱导产生肠道IFN-γ的T细胞频率与VirHRV相似，而AttHRV3x或2x疫苗效果较差。VirHRV攻击后针对轮状病毒腹泻的保护率与肠道产生IFN-γ的T细胞频率显著相关（r=0.97-1.0，p<0.005），表明它们在保护性免疫中的作用。
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1
IFN-gamma is the only anti-rotavirus cytokine found after in vitro stimulation of memory CD4+ T cells from mice immunized with a chimeric VP6 protein.γ干扰素是在用嵌合VP6蛋白免疫的小鼠的记忆CD4 + T细胞进行体外刺激后发现的唯一抗轮状病毒细胞因子。
Viral Immunol. 2007 Dec;20(4):571-84. doi: 10.1089/vim.2007.0055.
2
Identification of an immunodominant CD4+ T cell epitope in the VP6 protein of rotavirus following intranasal immunization of BALB/c mice.BALB/c小鼠经鼻内免疫后轮状病毒VP6蛋白中免疫显性CD4+ T细胞表位的鉴定
Virology. 2007 Jul 5;363(2):410-8. doi: 10.1016/j.virol.2007.01.041. Epub 2007 Mar 6.
3
Rotavirus infection alters peripheral T-cell homeostasis in children with acute diarrhea.轮状病毒感染会改变急性腹泻患儿外周血T细胞的稳态。
J Virol. 2007 Apr;81(8):3904-12. doi: 10.1128/JVI.01887-06. Epub 2007 Jan 31.
4
Association of gamma interferon and interleukin-17 production in intestinal CD4+ T cells with protection against rotavirus shedding in mice intranasally immunized with VP6 and the adjuvant LT(R192G).肠道CD4+ T细胞中γ干扰素和白细胞介素-17的产生与用VP6和佐剂LT(R192G)经鼻免疫的小鼠抵抗轮状病毒排泄的保护作用之间的关联
J Virol. 2007 Apr;81(8):3740-8. doi: 10.1128/JVI.01877-06. Epub 2007 Jan 24.
5
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Vet Immunol Immunopathol. 2007 Jan 15;115(1-2):56-67. doi: 10.1016/j.vetimm.2006.09.010. Epub 2006 Oct 27.
6
CD4+ T-cell memory: generation and multi-faceted roles for CD4+ T cells in protective immunity to influenza.CD4+ T细胞记忆：CD4+ T细胞在流感保护性免疫中的产生及多方面作用
Immunol Rev. 2006 Jun;211:8-22. doi: 10.1111/j.0105-2896.2006.00388.x.
7
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Eur J Immunol. 2006 Mar;36(3):583-92. doi: 10.1002/eji.200535520.
8
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Virology. 2006 Mar 15;346(2):338-47. doi: 10.1016/j.virol.2005.11.016. Epub 2006 Jan 10.
10
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