Lawson S, Rothwell L, Lambrecht B, Howes K, Venugopal K, Kaiser P
Institute for Animal Health, Compton, Berkshire, UK.
Dev Comp Immunol. 2001 Jan;25(1):69-82. doi: 10.1016/s0145-305x(00)00044-6.
Turkey and chicken interferon-gamma (IFN-gamma) share high identity (96.3% and 97% at the nucleotide and amino acid level, respectively). As such, we predicted that they would be functionally cross-reactive. To test this hypothesis, we produced recombinant turkey and chicken IFN-gamma, and compared their biological properties. Recombinant turkey and chicken IFN-gamma both induce HD11 cells (a chicken macrophage cell line) and LSTC-IAH30 cells (ALV-J-transformed turkey macrophages) to produce nitric oxide (NO), as measured in an avian IFN-gamma bioassay. Polyclonal and monoclonal antibodies, capable of neutralising the effect of chicken IFN-gamma on HD11 cells, were also shown to inhibit the activity of turkey IFN-gamma on these cells. The antibody neutralisation effect on both turkey and chicken IFN-gamma was shown by a significant reduction in NO production by HD11 cells when the neutralising antibodies were present in the bioassay. FACS analysis showed that HD11 and LSTC-IAH30 cells share some cell surface markers.
火鸡和鸡的γ干扰素（IFN-γ）具有高度同源性（核苷酸水平和氨基酸水平分别为96.3%和97%）。因此，我们推测它们在功能上具有交叉反应性。为了验证这一假设，我们制备了重组火鸡和鸡γ干扰素，并比较了它们的生物学特性。在禽类γ干扰素生物测定中，重组火鸡和鸡γ干扰素均能诱导HD11细胞（一种鸡巨噬细胞系）和LSTC-IAH30细胞（ALV-J转化的火鸡巨噬细胞）产生一氧化氮（NO）。能够中和鸡γ干扰素对HD11细胞作用的多克隆和单克隆抗体，也被证明能抑制火鸡γ干扰素对这些细胞的活性。当生物测定中存在中和抗体时，HD11细胞产生的NO显著减少，这表明抗体对火鸡和鸡γ干扰素均有中和作用。流式细胞术分析表明，HD11和LSTC-IAH30细胞具有一些共同的细胞表面标志物。
