Jansone I, Berzin V, Gribanov V, Gren E J
Nucleic Acids Res. 1979;6(5):1747-60. doi: 10.1093/nar/6.5.1747.
The functionally active fragments MS2 R(-53 leads to 6) and MS2 R(-53 leads to 3) comprising the regulatory region for the replicase cistron have been isolated from MS2 RNA-coat protein complex following T1 RNase digestion. In order to obtain shorter fragments, active in coat protein binding and initiation of translation, MS2 R(-53 leads to 6) was cleaved with S1 nuclease. The results indicate that S1 nuclease attacks the most susceptible loop regions of the two hairpin helices of MSZ R(-53) leads to 6). Among the three fragments which have been isolated, only MS2 R(-35/33 leads to 6) containing the intact hairpin (b) region with initiation codon AUG is active in the coat protein binding. Functional activity exerted by another polynucleotide MS R(-17 leads to 6) supports the assumption that specific binding with the coat protein is determined by the hairpin (b) region prior to the replicase cistron.
已从经T1核糖核酸酶消化后的MS2 RNA-外壳蛋白复合物中分离出包含复制酶顺反子调控区的功能活性片段MS2 R（-53至6）和MS2 R（-53至3）。为了获得在外壳蛋白结合和翻译起始方面具有活性的更短片段，用S1核酸酶切割了MS2 R（-53至6）。结果表明，S1核酸酶攻击MSZ R（-53）至6）的两个发夹螺旋中最敏感的环区。在已分离出的三个片段中，只有包含带有起始密码子AUG的完整发夹（b）区的MS2 R（-35/33至6）在外壳蛋白结合方面具有活性。另一种多核苷酸MS R（-17至6）发挥的功能活性支持了这样一种假设，即与外壳蛋白的特异性结合是由复制酶顺反子之前的发夹（b）区决定的。
