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噬菌体fr复制酶顺反子的调控区。III. 特定fr RNA片段的起始活性

The regulatory region of phage fr replicase cistron. III. Initiation activity of specific fr RNA fragments.

作者信息

Berzin V, Cielens I, Jansone I, Gren E J

出版信息

Nucleic Acids Res. 1982 Dec 11;10(23):7763-75. doi: 10.1093/nar/10.23.7763.

DOI:10.1093/nar/10.23.7763
PMID:6760130
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327044/
Abstract

RNA fragments from phage fr covering the complete or part of the replicase cistron initiation region have been used as templates in the formation of a ribosomal initiation complex in vitro. The results so obtained together with our earlier findings in a similar approach applied to fragments of the structurally related RNA from phage MS2 have allowed us to pinpoint the boundaries of the replicase cistron initiation region on phage RNA. A structural model of the above initiation region has been provided which shows that besides the minimal initiation region (comprises the Shine-Dalgarno sequence and initiator AUG), the flanking regions are also involved and are responsible for additional interactions with the ribosome. The flanking regions possibly contribute to the stability of specific contact between the ribosome and template realized by the minimal initiation region.

摘要

来自噬菌体fr、覆盖复制酶顺反子起始区域全部或部分的RNA片段已被用作体外形成核糖体起始复合物的模板。如此获得的结果，连同我们早期采用类似方法对来自噬菌体MS2的结构相关RNA片段的研究结果，使我们能够确定噬菌体RNA上复制酶顺反子起始区域的边界。已提供了上述起始区域的结构模型，该模型表明，除了最小起始区域（包含Shine-Dalgarno序列和起始密码子AUG）外，侧翼区域也参与其中，并负责与核糖体的额外相互作用。侧翼区域可能有助于最小起始区域实现的核糖体与模板之间特定接触的稳定性。

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本文引用的文献

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Use of the 'Perceptron' algorithm to distinguish translational initiation sites in E. coli.使用“感知器”算法区分大肠杆菌中的翻译起始位点。
Nucleic Acids Res. 1982 May 11;10(9):2997-3011. doi: 10.1093/nar/10.9.2997.
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Characterization of translational initiation sites in E. coli.大肠杆菌中转录起始位点的表征
Nucleic Acids Res. 1982 May 11;10(9):2971-96. doi: 10.1093/nar/10.9.2971.
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Mol Biol (Mosk). 1981 Jul-Aug;15(4):725-52.
5
The ribosome binding sites recognized by E. coli ribosomes have regions with signal character in both the leader and protein coding segments.大肠杆菌核糖体识别的核糖体结合位点在前导序列和蛋白质编码序列中都有具有信号特征的区域。
Nucleic Acids Res. 1980 Sep 11;8(17):3895-907. doi: 10.1093/nar/8.17.3895.
6
[Regulatory region of fr phage replicase cistron. II. Isolation and structure of specific fr RNA fragments].[噬菌体复制酶顺反子的调控区。II. 特异性fr RNA片段的分离与结构]
Mol Biol (Mosk). 1982 Sep-Oct;16(5):1109-15.
7
Polypeptide chain initiation: nucleotide sequences of the three ribosomal binding sites in bacteriophage R17 RNA.多肽链起始：噬菌体R17 RNA中三个核糖体结合位点的核苷酸序列
Nature. 1969 Dec 6;224(5223):957-64. doi: 10.1038/224957a0.
8
Direct physical evidence for secondary structure in an isolated fragment of R17 bacteriophage mRNA.R17噬菌体信使核糖核酸分离片段中二级结构的直接物理证据。
Nature. 1974 Mar 15;248(445):204-8. doi: 10.1038/248204a0.
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Free energy of imperfect nucleic acid helices. II. Small hairpin loops.不完全核酸螺旋的自由能。II. 小发夹环
J Mol Biol. 1973 Feb 5;73(4):497-511. doi: 10.1016/0022-2836(73)90096-x.
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Translation of R17 RNA by Escherichia coli ribosomes. Initiator transfer RNA-directed binding of 30 S subunits to the starting codon of the coat protein gene.大肠杆菌核糖体对R17 RNA的翻译。起始转运RNA引导30 S亚基与衣壳蛋白基因起始密码子的结合。
J Mol Biol. 1974 Nov 5;89(3):477-94. doi: 10.1016/0022-2836(74)90477-x.

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