van der Kooij P J, Persijn J P, van der Slik W, Korsten C B
Z Klin Chem Klin Biochem. 1975 Apr;13(4):149-55. doi: 10.1515/cclm.1975.13.4.149.
This paper deals with a new method for the determination of serum nucleotidase (EC 3.1.3.5). The assay is performed with cytidine-5'-monophosphate as substrate, followed by deamination of the generated cytidine. The principle of the method and the determination of the liberated ammonia by the Berthelot indophenol-reaction are comparable to the Persijn--van der Slik method in which adenosine-5'-monophosphate is used as substrate. The correlation between the results obtained with these two methods was found to be good; the new method has the advantage of higher sensitivity.
本文介绍了一种测定血清核苷酸酶（EC 3.1.3.5）的新方法。该测定以5'-单磷酸胞苷为底物进行，随后对生成的胞苷进行脱氨反应。该方法的原理以及通过伯塞洛特靛酚反应测定释放的氨，与以5'-单磷酸腺苷为底物的佩尔西恩 - 范德·斯利克方法类似。发现这两种方法所得结果之间的相关性良好；新方法具有更高灵敏度的优点。
