Hennon G, Plaquet R
Ann Biol Clin (Paris). 1975;33(1):21-8.
A semi-automated method for determining the 5'-nucleotidase activity in human serum for use with the Technicon Autoanalyzer I is described. Based upon the manual method of Persijn and Van Der Slik, adenosine formed by hydrolysis of 5'-AMP is deamined enzymatically and ammonia determined with the Berthelot reaction. The non specific alkaline phosphatases are inhibited by phenylphosphate. Concentrations of 5'-AMP and phenylphosphate are discussed. Nucleotidase activity is determined without dilution up to a value of 210 UI/liter. The precision is better than other manual or semi-automated methods in current use.
本文描述了一种用于Technicon自动分析仪I测定人血清中5'-核苷酸酶活性的半自动方法。该方法基于Persijn和Van Der Slik的手工方法，5'-AMP水解生成的腺苷经酶促脱氨，然后用伯塞洛特反应测定氨。非特异性碱性磷酸酶被苯磷酸盐抑制。文中讨论了5'-AMP和苯磷酸盐的浓度。核苷酸酶活性测定时无需稀释，最高可达210 UI/升。该方法的精密度优于目前使用的其他手工或半自动方法。
