Kole L, Chakrabarty D, Datta K, Bhattacharyya D
Division of Protein Engineering, Indian Institute of Chemical Biology, Calcutta, India.
Indian J Biochem Biophys. 2000 Apr;37(2):114-20.
A haemorrhagic toxin (VRR-12) from Vipera russelli russelli (Russell's viper) venom has been purified by ion-exchange chromatography on CM-Sephadex C-50 followed by size-exclusion HPLC to electrophoretically homogeneous state. It is a 12 kDa single polypeptide having 1 mole of Zn+2 ion. This toxin induces intense intestinal haemorrhage and to a lesser extent skeletal muscle haemorrhage in mice. It does not show detectable proteolytic and esterolytic activity with selected substrates under specified conditions, haemolytic and phospholipase activity. When VRR-12, preincubated with bivalent antiserum against Saw-scaled and Russell's viper venom or EDTA was injected, haemorrhagic activity was not reduced, on the other hand preincubation with phenylmethyl sulphonyl fluoride reduced the activity markedly. Biodistribution studies with 125I VRR-12 show that haemorrhagic manifestation by this toxin is not a direct function of the fraction of the totally administered toxin distributed to that tissue.
用CM - Sephadex C - 50离子交换色谱法，随后通过尺寸排阻高效液相色谱法，已将来自罗素蝰蛇（印度蝰蛇）毒液的一种出血毒素（VRR - 12）纯化至电泳均一状态。它是一种含有1摩尔Zn²⁺离子的12 kDa单多肽。这种毒素在小鼠中可引发强烈的肠道出血，并在较小程度上引发骨骼肌出血。在特定条件下，它对选定底物未表现出可检测到的蛋白水解和酯水解活性、溶血活性及磷脂酶活性。当注射预先与抗锯鳞蝰蛇和罗素蝰蛇毒液的二价抗血清或EDTA预孵育的VRR - 12时，出血活性并未降低，而与苯甲基磺酰氟预孵育则会显著降低活性。用¹²⁵I VRR - 12进行的生物分布研究表明，这种毒素的出血表现并非完全施用的毒素分配到该组织的部分的直接函数。
