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从圆斑蝰泰国亚种毒液中纯化具有抗癌作用的磷脂酶A(2)

Purification of a phospholipase A(2) from Daboia russelii siamensis venom with anticancer effects.

作者信息

Khunsap Suchitra, Pakmanee Narumol, Khow Orawan, Chanhome Lawan, Sitprija Visith, Suntravat Montamas, Lucena Sara E, Perez John C, Sánchez Elda E

出版信息

J Venom Res. 2011;2:42-51. Epub 2011 Oct 22.

PMID:22091349
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3210966/
Abstract

Venom phospholipases A2 (PLA(2)) are associated with neurotoxic, myotoxic, cardiotoxic, platelet aggregation, and edema activities. A PLA(2) (Drs-PLA(2)) was purified from Daboia russelii siamensis venom by a two-step purification procedure consisting of size-exclusion, followed by anion exchange high performance liquid chromatography (HPLC). The molecular weight of the Drs-PLA(2) was 13,679Da, which was determined by MALDI-TOF mass spectrometry. Its N-terminal amino acid sequence was homologous to basic PLA(2)s of viperid snake venoms. The Drs-PLA(2) had indirect hemolytic and anticoagulant activities, cytotoxic activity with a CC(50) of 65.8nM, and inhibited SK-MEL-28 cell migration with an IC(50) of 25.6nM. In addition, the Drs-PLA(2) inhibited the colonization of B16F10 cells in lungs of BALB/c mice by ∼65%.

摘要

蛇毒磷脂酶A2（PLA(2)）与神经毒性、肌毒性、心脏毒性、血小板聚集及水肿活性相关。通过两步纯化程序从圆斑蝰蛇毒中纯化出一种PLA(2)（Drs-PLA(2)），该程序包括尺寸排阻，随后进行阴离子交换高效液相色谱（HPLC）。Drs-PLA(2)的分子量为13,679Da，这是通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）测定的。其N端氨基酸序列与蝰蛇科蛇毒的碱性PLA(2)同源。Drs-PLA(2)具有间接溶血和抗凝活性，细胞毒性活性的半数致死浓度（CC(50)）为65.8nM，并且以25.6nM的半数抑制浓度（IC(50)）抑制SK-MEL-28细胞迁移。此外，Drs-PLA(2)使B16F10细胞在BALB/c小鼠肺中的定殖减少约65%。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d5e/3210966/138e7d1d7ead/JVR-02-042-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d5e/3210966/e12ebed09e9e/JVR-02-042-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d5e/3210966/71b9ac656499/JVR-02-042-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d5e/3210966/ee98b79c1197/JVR-02-042-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d5e/3210966/138e7d1d7ead/JVR-02-042-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d5e/3210966/e12ebed09e9e/JVR-02-042-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d5e/3210966/71b9ac656499/JVR-02-042-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d5e/3210966/ee98b79c1197/JVR-02-042-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d5e/3210966/138e7d1d7ead/JVR-02-042-g004.jpg

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