Saravanan R, Rajendran P, Thyagarajan S P, Smythe L D, Norris M A, Symonds M L, Dohnt M F
Department of Microbiology, Dr A. L. M. Post Graduate Institute of Basic Medical Sciences, Chennai, India.
Ann Trop Med Parasitol. 2000 Jul;94(5):503-6. doi: 10.1080/00034983.2000.11813569.
Leptospira were successfully isolated from the urine of an Indian patient who had been clinically diagnosed as having leptospirosis. In an attempt to determine the source of this infection, 28 rats (Rattus rattus) and 58 bandicoots (Bandicota bengalensis) living in the vicinity of the patient's home in Avadi, a suburban area of the city of Chennai (Madras), India, were then investigated. Each animal was checked for infection by microscopical examination of fresh and stained urine, serological analysis of serum, and the culture of urine and kidney samples. Direct, dark-field, observation of fresh urine samples and examination of urine samples after Fontana's silver staining were found to be the least sensitive of the tests used. The results of the serological microscopic agglutination test (MAT) indicated that four (14.3%) of the rats and nine (16.1%) of the bandicoots had significant agglutinins, predominantly for the serogroups icterohaemorrhagiae and autumnalis. Leptospira were isolated from at least one culture of samples from one rat and each of four bandicoots. Each of these rodent isolates and the human isolate were typed as Leptospira interrogans serovar autumnalis.
从一名临床诊断为钩端螺旋体病的印度患者尿液中成功分离出钩端螺旋体。为了确定该感染源，随后对生活在印度钦奈（马德拉斯）市郊区阿瓦迪患者家附近的28只大鼠（黑家鼠）和58只板齿鼠（孟加拉板齿鼠）进行了调查。通过对新鲜尿液和染色尿液进行显微镜检查、血清学分析以及尿液和肾脏样本培养，对每只动物进行感染检查。结果发现，直接暗视野观察新鲜尿液样本以及丰塔纳银染色后检查尿液样本是所用检测方法中最不敏感的。血清学显微镜凝集试验（MAT）结果表明，4只（14.3%）大鼠和9只（16.1%）板齿鼠有显著凝集素，主要针对黄疸出血群和秋季群。从1只大鼠和4只板齿鼠的至少一份样本培养物中分离出钩端螺旋体。这些啮齿动物分离株和人类分离株均被鉴定为问号钩端螺旋体秋季血清型。
