Priya C G, Hoogendijk K T, Berg Mvd, Rathinam S R, Ahmed A, Muthukkaruppan V R, Hartskeerl R A
Department of Immunology, Aravind Medical Research Foundation, No.1, Anna Nagar, Madurai - 625 020, Tamil Nadu, India.
J Postgrad Med. 2007 Oct-Dec;53(4):236-40. doi: 10.4103/0022-3859.37511.
To determine the seroprevalence of leptospires and to isolate Leptospira spp. from field rats and bandicoots in and around Madurai.
Thirteen rats and five bandicoots were trapped alive from fields in and around Madurai. Blood samples were tested for anti-leptospiral antibodies by microscopic agglutination test while the urine and kidney samples were used for isolation of leptospires. The isolated leptospires were tested for pathogenic status (13 degrees C test and PCR) followed by serological and genetic characterization.
Serology revealed the presence of anti-leptospiral antibodies in 58% (7/12) of field rats and leptospires were isolated from two urine and six kidney samples. The bandicoots were negative in both serology and culture. Analysis of the isolates from field rats revealed that all the isolates were pathogenic except for one, which was further confirmed by serological and genetic characterization. Six of the seven pathogenic isolates were identified as L. interrogans serogroup Autumnalis serovar Akiyami A and one as L. borgpetersenii serogroup Javanica serovar Veldrat Batavia 46.
Serology and isolation reveals that field rats are major natural carriers and shedders of leptospires in and around Madurai.
确定钩端螺旋体的血清流行率，并从马杜赖及其周边地区的田鼠和袋狸中分离出钩端螺旋体属。
从马杜赖及其周边地区的田野中活捉了13只田鼠和5只袋狸。通过显微镜凝集试验检测血样中的抗钩端螺旋体抗体，同时将尿液和肾脏样本用于分离钩端螺旋体。对分离出的钩端螺旋体进行致病性检测（13℃试验和聚合酶链反应），随后进行血清学和基因特征分析。
血清学检测显示，58%（7/12）的田鼠存在抗钩端螺旋体抗体，从两份尿液样本和六份肾脏样本中分离出了钩端螺旋体。袋狸的血清学检测和培养均为阴性。对来自田鼠的分离株分析表明，除一株外，所有分离株均具有致病性，血清学和基因特征分析进一步证实了这一点。七株致病性分离株中有六株被鉴定为问号钩端螺旋体秋季血清群秋波亚型，一株被鉴定为波摩那钩端螺旋体爪哇血清群威尔德布拉特巴达维亚46亚型。
血清学检测和分离结果表明，田鼠是马杜赖及其周边地区钩端螺旋体的主要自然携带者和排泄者。
