Meldrum D
Department of Electrical Engineering, Genomation Laboratory, University of Washington, Seattle, Washington 98195-2500, USA.
Genome Res. 2000 Sep;10(9):1288-303. doi: 10.1101/gr.157400.
Automation for genomics has enabled a 43-fold increase in the total finished human genomic sequence in the world in the past four years. This is the second half of a two-part, noncomprehensive review that presents an overview of different types of automation equipment used in genome sequencing. The first part of the review, published in the previous issue, focused on automated procedures used to prepare DNA for sequencing or analysis. This second part of the review presents a look at available DNA sequencers and array technology and concludes with a look at future technologies. Alternate sequencing technologies including mass spectrometry, biochips, and single molecule analysis are included in this review.
基因组学自动化技术使得过去四年里全球已完成的人类基因组序列总量增长了43倍。这是一篇分两部分的非全面综述的后半部分，该综述概述了基因组测序中使用的不同类型的自动化设备。综述的第一部分发表在上一期，重点介绍了用于制备DNA进行测序或分析的自动化程序。综述的第二部分介绍了现有的DNA测序仪和阵列技术，并展望了未来技术。本综述还包括了质谱分析、生物芯片和单分子分析等替代测序技术。
