Tegally Houriiyah, San James Emmanuel, Giandhari Jennifer, de Oliveira Tulio
Kwazulu-Natal Research and Innovation Sequencing Platform (KRISP), College of Health Sciences, K-RITH Tower Building, Nelson R Mandela School of Medicine, University of KwaZulu-Natal, 719 Umbilo Road, Durban, South Africa.
Department of Global Health, University of Washington, 908 Jefferson Street, 13th Floor, Seattle, WA, 98104, USA.
BMC Genomics. 2020 Oct 20;21(1):729. doi: 10.1186/s12864-020-07137-1.
In research and clinical genomics laboratories today, sample preparation is the bottleneck of experiments, particularly when it comes to high-throughput next generation sequencing (NGS). More genomics laboratories are now considering liquid-handling automation to make the sequencing workflow more efficient and cost effective. The question remains as to its suitability and return on investment. A number of points need to be carefully considered before introducing robots into biological laboratories. Here, we describe the state-of-the-art technology of both sophisticated and do-it-yourself (DIY) robotic liquid-handlers and provide a practical review of the motivation, implications and requirements of laboratory automation for genome sequencing experiments.
在当今的研究和临床基因组学实验室中，样本制备是实验的瓶颈，尤其是在进行高通量下一代测序（NGS）时。现在，越来越多的基因组学实验室正在考虑采用液体处理自动化技术，以使测序工作流程更高效且更具成本效益。但其适用性和投资回报率仍是问题。在将机器人引入生物实验室之前，有许多要点需要仔细考虑。在此，我们描述了先进的和自行组装（DIY）的机器人液体处理仪的最新技术，并对基因组测序实验的实验室自动化的动机、影响和要求进行了实际评估。
