Brown L S
Department of Physiology and Biophysics, University of California, 92697-4560, Irvine, CA, USA.
Biochim Biophys Acta. 2000 Aug 30;1460(1):49-59. doi: 10.1016/s0005-2728(00)00129-8.
As a result of the number of new high-resolution structures of the pigment and some of its photointermediates, a realistic model for the functioning of bacteriorhodopsin seems to be finally emerging. However, lack of structural information for some of the key functional states, and contradictions between some published structural models, argue for the use of the synthetic approach, one that includes use of data from both crystallographic and mutagenesis studies. The role of mutagenesis in this synthetic approach falls into two categories. First, to provide additional structural information, and second, to test the predictions of structural models by studying mutant phenotypes. This review urges critical comparisons of the structural and mutagenesis data, as there are problems with their selective and indiscriminate use.
由于色素及其一些光中间体的新高分辨率结构数量众多，一种关于细菌视紫红质功能的现实模型似乎终于浮现出来。然而，一些关键功能状态缺乏结构信息，且一些已发表的结构模型之间存在矛盾，这表明需要采用综合方法，即一种包括使用晶体学和诱变研究数据的方法。诱变在这种综合方法中的作用可分为两类。第一，提供额外的结构信息；第二，通过研究突变体表型来检验结构模型的预测。这篇综述敦促对结构和诱变数据进行批判性比较，因为在选择性和不加区分地使用这些数据时存在问题。
