Kandori H
Department of Biophysics, Graduate School of Science, Kyoto University, Sakyo-ku, 606-8502, Kyoto, Japan.
Biochim Biophys Acta. 2000 Aug 30;1460(1):177-91. doi: 10.1016/s0005-2728(00)00138-9.
Internal water molecules are considered to play a crucial role in the functional processes of proton pump proteins. They may participate in hydrogen-bonding networks inside proteins that constitute proton pathways. In addition, they could participate in the switch reaction by mediating an essential proton transfer at the active site. Nevertheless, little has been known about the structure and function of internal water molecules in such proteins. Recent progress in infrared spectroscopy and X-ray crystallography provided new information on water molecules inside bacteriorhodopsin, the light-driven proton pump. The accumulated knowledge on bacteriorhodopsin in the last decade of the 20th century will lead to a realistic picture of internal water molecules at work in the 21st century. In this review, I describe how the role of water molecules has been studied in bacteriorhodopsin, and what should be known about the role of water molecules in the future.
内部水分子被认为在质子泵蛋白的功能过程中起着至关重要的作用。它们可能参与构成质子通道的蛋白质内部的氢键网络。此外，它们可以通过介导活性位点的关键质子转移来参与开关反应。然而，对于此类蛋白质中内部水分子的结构和功能却知之甚少。红外光谱学和X射线晶体学的最新进展提供了关于光驱动质子泵细菌视紫红质内部水分子的新信息。20世纪最后十年积累的关于细菌视紫红质的知识将为21世纪工作中的内部水分子描绘出一幅真实的图景。在这篇综述中，我描述了如何在细菌视紫红质中研究水分子的作用，以及未来关于水分子的作用应该了解些什么。
