Arruda A F, Campiglia A D
Department of Chemistry, North Dakota State University, Fargo 58105-5516, USA.
Analyst. 1997 Jun;122(6):559-62. doi: 10.1039/a700309i.
A simple, rapid and sensitive method for the determination of indomethacin was developed based on solid-surface room-temperature phosphorimetry. Phosphorescence emission was induced by the indomethacin hydrolysis reaction in a basic medium. Chromatography paper treated for background reduction was employed as a solid substrate. The use of four heavy-atom salts and sodium dodecyl sulfate was optimized to estimate an absolute limit of detection at the subnanogram level. A calibration curve with a linear dynamic range extending over two orders of magnitude (3.0 x 10(-7)-1.0 x 10(-4) mol dm-3) was obtained. The recovery of the method (98.4 +/- 6.8%) was tested using commercial formulations containing indomethacin as the only anti-inflammatory agent.
基于固体表面室温磷光法开发了一种简单、快速且灵敏的吲哚美辛测定方法。在碱性介质中，吲哚美辛水解反应诱导磷光发射。经处理以降低背景的层析纸用作固体基质。对四种重原子盐和十二烷基硫酸钠的使用进行了优化，以估计亚纳克级的绝对检测限。获得了线性动态范围跨越两个数量级（3.0×10⁻⁷ - 1.0×10⁻⁴ mol dm⁻³）的校准曲线。使用仅含吲哚美辛作为唯一抗炎剂的商业制剂测试了该方法的回收率（98.4±6.8%）。
