Gan W B, Grutzendler J, Wong W T, Wong R O, Lichtman J W
Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Neuron. 2000 Aug;27(2):219-25. doi: 10.1016/s0896-6273(00)00031-3.
We describe a technique for rapid labeling of a large number of cells in the nervous system with many different colors. By delivering lipophilic dye-coated particles to neuronal preparations with a "gene gun," individual neurons and glia whose membranes are contacted by the particles are quickly labeled. Using particles that are each coated with different combinations of various lipophilic dyes, many cells within a complex neuronal network can be simultaneously labeled with a wide variety of colors. This approach is most effective in living material but also labels previously fixed material. In living material, labeled neurons continue to show normal synaptic responses and undergo dendritic remodeling. This technique is thus useful for studying structural plasticity of neuronal circuits in living preparations. In addition, the Golgi-like labeling of neurons with many different colors provides a novel way to study neuronal connectivity.
我们描述了一种用多种不同颜色快速标记神经系统中大量细胞的技术。通过用“基因枪”将亲脂性染料包被的颗粒递送至神经元制剂，与颗粒接触到其膜的单个神经元和神经胶质细胞会被快速标记。使用分别包被有各种亲脂性染料不同组合的颗粒，复杂神经元网络中的许多细胞可同时用多种颜色进行标记。这种方法在活材料中最为有效，但也能标记先前固定的材料。在活材料中，被标记的神经元继续表现出正常的突触反应并经历树突重塑。因此，该技术对于研究活体制剂中神经元回路的结构可塑性很有用。此外，用多种不同颜色对神经元进行类似高尔基染色的标记为研究神经元连接性提供了一种新方法。
