用于神经系统结构和功能研究的染料的弹道递送。
Ballistic delivery of dyes for structural and functional studies of the nervous system.
作者信息
Gan Wen-Biao, Grutzendler Jaime, Wong Rachel O, Lichtman Jeff W
出版信息
Cold Spring Harb Protoc. 2009 Apr;2009(4):pdb.prot5202. doi: 10.1101/pdb.prot5202.
Abstract
This protocol describes detailed procedures for rapid labeling of cells in a variety of preparations by means of particle-mediated ballistic (i.e., Gene Gun) delivery of fluorescent dyes. The method has been used for rapid labeling of cells with either lipid- or water-soluble dyes, in a variety of preparations at different ages. Tissue preparations include fixed mouse brain slices (described here), cell cultures, and tissue explants. This ballistic labeling technique is useful for studying neuronal connectivity, function, and pathology in the nervous system of living as well as fixed specimens.
摘要
本方案描述了通过粒子介导的弹道式（即基因枪）荧光染料递送，对多种制剂中的细胞进行快速标记的详细程序。该方法已用于用脂溶性或水溶性染料对不同年龄的多种制剂中的细胞进行快速标记。组织制剂包括固定的小鼠脑切片（此处所述）、细胞培养物和组织外植体。这种弹道式标记技术可用于研究活体和固定标本神经系统中的神经元连接、功能和病理学。
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本文引用的文献
1
The synaptic remodeling between regenerated perforant pathway and granule cells in slice culture.切片培养中再生的穿通通路与颗粒细胞之间的突触重构。
Cell Mol Neurobiol. 2010 Mar;30(2):309-16. doi: 10.1007/s10571-009-9454-7. Epub 2009 Sep 16.
2
Synaptogenesis in the developing mouse visual cortex.发育中的小鼠视觉皮层中的突触发生。
Brain Res Bull. 2010 Jan 15;81(1):107-13. doi: 10.1016/j.brainresbull.2009.08.028.
3
Combination of diOlistic labeling with retrograde tract tracing and immunohistochemistry.双示踪与免疫组织化学结合逆行追踪。
J Neurosci Methods. 2009 Nov 15;184(2):332-6. doi: 10.1016/j.jneumeth.2009.08.016. Epub 2009 Aug 25.
4
Uniquely hominid features of adult human astrocytes.成年人类星形胶质细胞独特的人科特征。
J Neurosci. 2009 Mar 11;29(10):3276-87. doi: 10.1523/JNEUROSCI.4707-08.2009.
5
A role for local calcium signaling in rapid synaptic partner selection by dendritic filopodia.局部钙信号在树突丝状伪足快速突触伙伴选择中的作用。
Neuron. 2008 Jul 31;59(2):253-60. doi: 10.1016/j.neuron.2008.05.025.
6
Loss of astrocytic domain organization in the epileptic brain.癫痫大脑中星形胶质细胞结构域组织的丧失。
J Neurosci. 2008 Mar 26;28(13):3264-76. doi: 10.1523/JNEUROSCI.4980-07.2008.
7
Morphological properties of mouse retinal ganglion cells during postnatal development.出生后发育过程中小鼠视网膜神经节细胞的形态学特性
J Comp Neurol. 2007 Aug 20;503(6):803-14. doi: 10.1002/cne.21429.
8
Troponin C-based biosensors: a new family of genetically encoded indicators for in vivo calcium imaging in the nervous system.基于肌钙蛋白C的生物传感器：用于神经系统体内钙成像的新型基因编码指示剂家族。
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9
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10
Organization of spines on the dendrites of Purkinje cells.浦肯野细胞树突棘的组织结构。
Proc Natl Acad Sci U S A. 2006 Jan 31;103(5):1575-80. doi: 10.1073/pnas.0507884103. Epub 2006 Jan 19.
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