Bica A, Farinha A, Blume H, Barbosa C M
Laboratório de Estudos Farmacêuticos, Lisbon, Portugal.
J Chromatogr A. 2000 Aug 11;889(1-2):135-41. doi: 10.1016/s0021-9673(00)00348-4.
A reversed-phase high-performance liquid chromatographic method that enables the determination of clonixin in human plasma and urine samples is described. Recovery of the drug was over 87.6 and 80.7% for plasma and urine, respectively. The limit of quantitation of the method was established as 10 ng/ml in plasma and 20 ng/ml in urine samples, with RSDs of less than 11.1%. The applicability of the method was further assessed by determining the plasma concentrations time course of clonixin in six healthy volunteers after single oral dose administration of 150 and 300 mg of clonixin and Clonix.
本文描述了一种反相高效液相色谱法，该方法可用于测定人血浆和尿液样本中的氯尼辛。该药物在血浆和尿液中的回收率分别超过87.6%和80.7%。该方法的定量限设定为血浆中10 ng/ml，尿液样本中20 ng/ml，相对标准偏差小于11.1%。通过测定6名健康志愿者单次口服150 mg和300 mg氯尼辛及氯尼辛制剂后血浆中氯尼辛的浓度-时间过程，进一步评估了该方法的适用性。
