Delbeke F T, Landuyt J, Debackere M
Vakgroep Farmacologie, Farmacie on Toxicologie, Faculteit Diergeneeskunde, Universiteit Gent, Belgium.
J Chromatogr. 1993 Nov 24;621(2):209-14. doi: 10.1016/0378-4347(93)80097-n.
A high-performance liquid chromatographic method to measure plasma and urinary alclofenac levels in equine biofluids is described. Isolation of the drug from plasma is achieved using liquid-liquid extraction with diethyl ether. Reversed-phase C18 solid phase extraction is used for the extraction of free and conjugated alclofenac from urine. The reproducibility and accuracy of the method were well within acceptable limits over the concentration ranges 0-10 and 0-20 micrograms/ml, respectively, for plasma and urine. Starting with 2 ml of plasma, a concentration of 0.1 microgram/ml could easily be measured; the limit of quantification in urine (0.5 ml) was 1 microgram/ml. Hydrolysis of urine with strong alkali resulted in the decomposition of alclofenac. A pharmacokinetic profile of alclofenac in the horse is shown.
本文描述了一种用于测量马生物流体中血浆和尿液中阿氯芬酸水平的高效液相色谱法。通过用乙醚进行液-液萃取从血浆中分离药物。反相C18固相萃取用于从尿液中萃取游离和结合的阿氯芬酸。该方法在血浆和尿液浓度范围分别为0-10微克/毫升和0-20微克/毫升时的重现性和准确性均在可接受范围内。从2毫升血浆开始，0.1微克/毫升的浓度很容易测量；尿液（0.5毫升）中的定量限为1微克/毫升。用强碱水解尿液会导致阿氯芬酸分解。展示了阿氯芬酸在马体内的药代动力学概况。
