Uchihara T, Nakamura A, Yamazaki M, Mori O
Department of Neuropathology, Tokyo Metropolitan Institute for Neuroscience, Japan.
Acta Neuropathol. 2000 Oct;100(4):385-9. doi: 10.1007/s004010000186.
Thiazin red (TR), a fluorochrome that has an affinity to fibrillary structures such as neurofibrillary tangles (NFTs) or senile plaques, was utilized to investigate assembly of tau protein into fibrils in tau-immunopositive neocortical neurons of corticobasal degeneration (CBD) and of Alzheimer's disease (AD). Double fluorescence with anti-paired helical filament monoclonal antibody (AT8) and TR was followed by either the Gallyas or Bodian silver impregnation method, which enabled a comparison of the staining features by three different methods on the same neuron. NFTs of AD were uniformly stained by TR and Gallyas method. Most of tau-immunopositive neurons of CBD were similarly stained by Gallyas method but barely or only weakly by TR or Bodian method, suggesting that tau in neocortical neurons of CBD is less liable to form fibrillary structures than in those of AD, easily distinguishable by TR staining. Clarifying the process of tau assembly using this fluorochrome will give a clue to understanding mechanisms of tau deposition, which may be different in various neurological disorders.
噻嗪红（TR）是一种对神经原纤维缠结（NFTs）或老年斑等纤维状结构具有亲和力的荧光染料，用于研究皮质基底节变性（CBD）和阿尔茨海默病（AD）的tau免疫阳性新皮质神经元中tau蛋白组装成纤维的情况。用抗双螺旋丝单克隆抗体（AT8）和TR进行双重荧光标记，随后采用Gallyas或Bodian银浸染法，这使得能够在同一神经元上通过三种不同方法比较染色特征。AD的NFTs经TR和Gallyas法均匀染色。CBD的大多数tau免疫阳性神经元经Gallyas法染色类似，但经TR或Bodian法染色很少或仅微弱染色，这表明CBD新皮质神经元中的tau比AD中的tau更不易形成纤维状结构，通过TR染色很容易区分。使用这种荧光染料阐明tau组装过程将为理解tau沉积机制提供线索，而tau沉积机制在各种神经疾病中可能不同。
