Uchihara T, Mizusawa H, Tsuchiya K, Kondo H, Oda T, Ikeda K
Department of Neurology, Tokyo Medical and Dental University, Japan.
Acta Neuropathol. 1998 Dec;96(6):553-7. doi: 10.1007/s004010050934.
To clarify different features of cytoskeletal pathology in neocortical neurons between corticobasal degeneration (CBD) and Alzheimer's disease (AD), the relationship between neurofibrillary tangles (NFTs), which are defined as a fibrillary structure stained by the Bodian method, and tau-immunopositive neurons in layers II-III of the premotor cortex was assessed by sequential staining for tau immunohistochemistry followed by the Bodian method on the same section. In AD brains, tau-like immunoreactivity in neurons was uniformly colocalized with Bodian-positive NFTs. In CBD brains, however, tau-immunopositive neurons could be classified into three different types: (1) those not stained at all by the Bodian method (diffuse cytoplasmic type), which represented the majority of these immunopositive neurons, (2) some partly stained by the Bodian method (mixed type), and (3) a few for which argyrophilia demonstrated by the Bodian method was completely colocalized with immunoreactivity (NFT type). This discrepancy between tau-like immunoreactivity and Bodian method is characteristic of CBD and suggests that the tau molecule is less liable to form argyrophilic fibrils in neocortical neurons of CBD.
为了阐明皮质基底节变性（CBD）和阿尔茨海默病（AD）新皮质神经元细胞骨架病理的不同特征，通过在同一脑切片上依次进行tau免疫组化染色及博迪安法染色，评估了运动前皮质II - III层中被定义为经博迪安法染色呈纤维状结构的神经原纤维缠结（NFTs）与tau免疫阳性神经元之间的关系。在AD脑内，神经元中tau样免疫反应性与博迪安阳性NFTs完全共定位。然而，在CBD脑内，tau免疫阳性神经元可分为三种不同类型：（1）完全不被博迪安法染色的神经元（弥漫性胞质型），这类免疫阳性神经元占大多数；（2）部分被博迪安法染色的神经元（混合型）；（3）少数经博迪安法显示嗜银性且与免疫反应性完全共定位的神经元（NFT型）。tau样免疫反应性与博迪安法之间的这种差异是CBD的特征，提示在CBD新皮质神经元中tau分子较不易形成嗜银性纤维。
