聚球藻菌株PCC 7942的glnN产物（谷氨酰胺合成酶III）有助于从长期的氮素缺绿症中恢复。
The Synechococcus strain PCC 7942 glnN product (glutamine synthetase III) helps recovery from prolonged nitrogen chlorosis.
作者信息
Sauer J, Dirmeier U, Forchhammer K
机构信息
Institut für Mikrobiologie und Molekularbiologie der Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
出版信息
J Bacteriol. 2000 Oct;182(19):5615-9. doi: 10.1128/JB.182.19.5615-5619.2000.
Abstract
We report the cloning and sequencing of the glnN gene encoding a class III glutamine synthetase from the cyanobacterium Synechococcus strain PCC 7942. Mapping of the transcriptional start site revealed a DNA sequence in the promoter region that resembles an imperfect NtcA binding motif. Expression of glnN is impaired in NtcA- and P(II)-deficient mutants. The only parameter which was negatively affected in the glnN mutant compared to the wild type was the recovery rate of prolonged nitrogen-starved cells with low concentrations of combined nitrogen.
摘要
我们报道了来自蓝藻聚球藻菌株PCC 7942的编码III类谷氨酰胺合成酶的glnN基因的克隆和测序。转录起始位点的定位揭示了启动子区域中的一个DNA序列，该序列类似于一个不完美的NtcA结合基序。在NtcA和P(II)缺陷型突变体中，glnN的表达受损。与野生型相比，glnN突变体中唯一受到负面影响的参数是低浓度化合态氮长时间饥饿细胞的恢复率。
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