肌联蛋白PEVK区域的序列及力学意义。

Sequence and mechanical implications of titin's PEVK region.

作者信息

Greaser M L, Wang S M, Berri M, Mozdziak P, Kumazawa Y

机构信息

Muscle Biology Laboratory, University of Wisconsin-Madison, USA.

出版信息

Adv Exp Med Biol. 2000;481:53-63; discussion 64-6, 107-10. doi: 10.1007/978-1-4615-4267-4_4.

DOI:10.1007/978-1-4615-4267-4_4

PMID:10987066

Abstract

A widely used titin monoclonal antibody (9D10) was epitope mapped to the PEVK region in the I-band portion of titin. Sequence analysis of the titin PEVK region revealed a large number of 28 amino acid modules (termed "PPAK" repeats) alternating with glutamic acid rich segments. Species differences in cardiac rest tension could not be ascribed to differences in the PEVK length of the N2B titin isoform. The low rest tension generated by dog cardiac muscle also does not appear to be explained by the N2 and PEVK segment lengths in the N2A titin isoform.

摘要

一种广泛使用的肌联蛋白单克隆抗体（9D10）的表位被定位到肌联蛋白I带部分的PEVK区域。肌联蛋白PEVK区域的序列分析显示，大量28个氨基酸的模块（称为“PPAK”重复序列）与富含谷氨酸的片段交替出现。心脏静息张力的物种差异不能归因于N2B肌联蛋白同工型的PEVK长度差异。犬心肌产生的低静息张力似乎也不能用N2A肌联蛋白同工型中的N2和PEVK片段长度来解释。