Seidman J G, Schmidt F J, Foss K, McClain W H
Cell. 1975 Aug;5(4):389-400. doi: 10.1016/0092-8674(75)90058-6.
The conversion of precursor RNA into bacteriophage T4 proline and serine transfer RNAs includes two steps for the enzymatic removal of nucleotides from the 3' ends of RNA chains. Neither of these steps occur following infection of a mutant of Escherichia coli that was previously shown to block the suppressor function of T4 serine transfer RNA. Cell-free extracts of this mutant are furthermore deficient in a wild type enzyme activity that removes nucleotides from the 3' ends of one of the RNA chains described above. The relation of this enzyme to other 3' ribonucleases is not known. We subsequently examined the mutant for its ability to support the biosynthesis of other bacteriophage transfer RNAs. In one instance that is analogous to the proline-serine precursor RNA, maturation of the precursor RNA was blocked during infection of mutant cells. In another instance, precursor RNA maturation was normal, even though this involved the removal of 3'nucleotides. These observations point to the possible existence of at least two 3' ribonucleases for the biosynthesis of transfer RNAs.
前体RNA转化为噬菌体T4脯氨酸和丝氨酸转运RNA包括两个从RNA链3'末端酶促去除核苷酸的步骤。在感染先前已证明会阻断T4丝氨酸转运RNA抑制功能的大肠杆菌突变体后，这两个步骤均不会发生。此外，该突变体的无细胞提取物缺乏一种野生型酶活性，该活性可从上述RNA链之一的3'末端去除核苷酸。这种酶与其他3'核糖核酸酶的关系尚不清楚。我们随后检测了该突变体支持其他噬菌体转运RNA生物合成的能力。在一个类似于脯氨酸 - 丝氨酸前体RNA的例子中，突变体细胞感染期间前体RNA的成熟被阻断。在另一个例子中，前体RNA成熟正常，尽管这涉及3'核苷酸的去除。这些观察结果表明，转运RNA生物合成可能至少存在两种3'核糖核酸酶。
