McClain W H, Guthrie C, Barrell B G
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3703-7. doi: 10.1073/pnas.69.12.3703.
Bacteriophage T4 induces the synthesis of eight transfer RNAs upon infection of E. coli. The tRNAs are easily detected and resolved into pure species by polyacrylamide gel electrophoresis of RNA labeled with (32)P after T4 infection. Two-dimensional fingerprints of RNase T(1) products derived from individual gel bands give patterns characteristic of single tRNAs. Furthermore, the T(1) digest of each gel band has a single oligonucleotide that contains the minor nucleotides Tp and Psip, a characteristic feature of all known tRNAs. Four larger RNAs are also seen in the polyacrylamide gels. Fingerprint and genetic analyses demonstrate that these molecules are related to the tRNAs, but the exact nature of this relationship is not known.
噬菌体T4感染大肠杆菌后会诱导合成8种转移RNA。这些tRNA很容易被检测到，并且在T4感染后用（32）P标记RNA，通过聚丙烯酰胺凝胶电泳可将其分离为纯的种类。从各个凝胶条带衍生的核糖核酸酶T（1）产物的二维指纹图谱给出了单个tRNA的特征模式。此外，每个凝胶条带的T（1）消化产物都有一个包含稀有核苷酸Tp和Psip的单一寡核苷酸，这是所有已知tRNA的一个特征。在聚丙烯酰胺凝胶中还可以看到4种较大的RNA。指纹图谱和遗传学分析表明这些分子与tRNA有关，但这种关系的确切性质尚不清楚。
