Crabeel M, Charlier D, Cunin R, Boyen A, Glansdorff N, Piérard A
J Bacteriol. 1975 Sep;123(3):898-904. doi: 10.1128/jb.123.3.898-904.1975.
The accumulation or ornithine, citrulline, and possibly acetylornithine by Escherichia coli K-12 arginineless mutants provided with acetylarginine as source of arginine causes severe growth inhibition. This occurs under conditions where comparable derivatives of E. coli W (Bollon and Vogel, 1973) show little or no growth inhibition. The same conditions, which have been reported to cause noncorrelative synthesis of acetylornithinase and argininosuccinase in E. coli W (Bollon and Vogel, 1973), do not alter the correlative pattern of enzyme synthesis observed in E. coli K-12. Moreover, previously reported effects of ornithine and citrulline on repression of the arginine regulon in E. coli W are not observed in the K-12 strains examined. The bearing of these observations on possible differences between the mechanism of enzyme repression operating in the two types of strains cannot yet be fully evaluated; it is, however, clear that considerable care should be exercised before extrapolating the results obtained with one type of strain to the other one. The particularly strong inhibition of acetylarginine utilization exerted by ornithine in E. coli K-12 allows the forward selection of several classes of arginine auxotrophs from strains deficient in carbamoylphosphate biosynthesis and thus capable of ornithine accumulation. Possible applications of this technique to the genetic analysis of the bipolar argECBH operon are discussed.
用乙酰精氨酸作为精氨酸来源时，大肠杆菌K - 12无精氨酸突变体积累鸟氨酸、瓜氨酸以及可能的乙酰鸟氨酸会导致严重的生长抑制。这种情况发生的条件下，大肠杆菌W的可比衍生物（博隆和沃格尔，1973年）几乎没有或没有生长抑制。同样的条件，据报道在大肠杆菌W中会导致乙酰鸟氨酸酶和精氨琥珀酸酶的非相关合成（博隆和沃格尔，1973年），但不会改变在大肠杆菌K - 12中观察到的酶合成相关模式。此外，在检测的K - 12菌株中未观察到先前报道的鸟氨酸和瓜氨酸对大肠杆菌W中精氨酸调节子阻遏的影响。这些观察结果对于两种菌株中酶阻遏机制可能存在的差异的影响尚未能完全评估；然而，很明显，在将一种菌株获得的结果外推到另一种菌株之前应格外谨慎。在大肠杆菌K - 12中，鸟氨酸对乙酰精氨酸利用的特别强烈抑制使得能够从缺乏氨甲酰磷酸生物合成因而能够积累鸟氨酸的菌株中正向选择几类精氨酸营养缺陷型。讨论了该技术在双极argECBH操纵子遗传分析中的可能应用。
