细胞表面在细菌交配中的作用：大肠杆菌R+菌株中供体完整粘肽对表面排斥表达的要求。
Role of the cell surface in bacterial mating: requirement for intact mucopeptide in donors for the expression of surface exclusion in R+ strains of Escherichia coli.
作者信息
Beard J P, Bishop S F
出版信息
J Bacteriol. 1975 Sep;123(3):916-20. doi: 10.1128/jb.123.3.916-920.1975.
Abstract
Two derivatives of the F-like R factor R1drd19 carrying mutually exclusive resistance determinants were used to study the role of the mucopeptide in the expression of conjugal functions. The use of metabolically active penicillin spheroplasts in R+ times R- matings had no effect on the ability of the cells to donate or accept a plasmid. However, in R+ times R+ matings it was found that surface exclusion was totally abolished if the donor, but not the recipient, was a spheroplast. This result implies that the traS gene, expressed by the excluding plasmid, is dependent for its action on an intact mucopeptide layer in the donor cell, and that this interaction is independent of the transfer ability of the excluding plasmid.
摘要
利用携带相互排斥抗性决定簇的F样R因子R1drd19的两种衍生物，研究了粘肽在接合功能表达中的作用。在R⁺×R⁻交配中使用代谢活跃的青霉素原生质体，对细胞捐赠或接受质粒的能力没有影响。然而，在R⁺×R⁺交配中发现，如果供体而非受体是原生质体，则表面排斥完全消除。这一结果表明，由排斥质粒表达的traS基因的作用依赖于供体细胞中完整的粘肽层，并且这种相互作用与排斥质粒的转移能力无关。
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Role of the cell surface in bacterial mating: requirement for intact mucopeptide in donors for the expression of surface exclusion in R+ strains of Escherichia coli.细胞表面在细菌交配中的作用：大肠杆菌R+菌株中供体完整粘肽对表面排斥表达的要求。
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