通过接合作用将R1drd19质粒从大肠杆菌J53转移至三叶草根瘤菌。
Transfer of R1drd19 plasmid from Escherichia coli J53 to Rhizobium trifolii by conjugation.
作者信息
Kowalczuk E, Lorkiewicz Z
出版信息
Acta Microbiol Pol. 1977;26(1):9-18.
Rldrd19 plasmid was transferred by conjugation from Escherichia coli J53 to Rhizobium trifolii T24, T26 and 24XSM strains with frequency 10(-3) to 10(-5). The R. trifolii exconjugants carrying Rldrd19 were in turn able to transfer the R factor by conjugation to other R. trifolii strains. Rldrd19 was maintained stably in R. trifolii. R. trifolii 24XSM, T26 and 14M were also found to harbor an endogenous plasmid (molecular weight 5.5 megadaltons) with undetermined as yet properties. Ridrd19 could be stably maintained in the same cell together with the endogenous plasmid.
Rldrd19质粒通过接合作用从大肠杆菌J53转移至三叶草根瘤菌T24、T26和24XSM菌株，转移频率为10(-3)至10(-5)。携带Rldrd19的三叶草根瘤菌接合子反过来又能够通过接合作用将R因子转移至其他三叶草根瘤菌菌株。Rldrd19在三叶草根瘤菌中稳定维持。还发现三叶草根瘤菌24XSM、T26和14M含有一种内源质粒（分子量5.5兆道尔顿），其性质尚未确定。Ridrd19可以与内源质粒一起在同一细胞中稳定维持。
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