Willetts N
J Bacteriol. 1974 Jun;118(3):778-82. doi: 10.1128/jb.118.3.778-782.1974.
A series of Hfr deletion strains carrying deletions extending different distances into the integrated F factor have been used to map loci for surface exclusion (traS) and for incompatibility (inc) on the Escherichia coli K-12 sex factor F. traS mapped between traG and traD. It forms a part of the large operon, including all the known transfer genes except traJ, and is co-controlled with these. The product of traS is not required for formation of the F pilus. inc mapped between the phi(R) (11) locus and the origin of transfer; it is therefore one of the earliest loci transferred during conjugation.
一系列携带缺失片段的高频重组(Hfr)缺失菌株已被用于绘制大肠杆菌K-12性因子F上表面排斥(traS)和不相容性(inc)位点的图谱,这些缺失片段向整合的F因子内延伸不同距离。traS定位于traG和traD之间。它是一个大操纵子的一部分,包括除traJ之外的所有已知转移基因,并与这些基因共同受调控。形成F菌毛不需要traS的产物。inc定位于φ(R)(11)位点和转移起点之间;因此,它是接合过程中最早转移的位点之一。