Kato L, Ishaque M, Walsh G P
Microbios. 1975;12(47-48):41-50.
The bacilli were isolated from granulomata harvested from armadillos. Cytochrome systems in whole cell suspensions as well as in cell-free extracts were examined spectrophotometrically. The intact cells contained cytochromes of the a+b3, b and c type which were found to be present mainly in the reduced form. The cytochrome systems in cell-free extracts of M. leprae were in the oxidized form but contained the same type of cytochromes as the intact bacteria. The presence of cytochromes was easily detectable in the anaerobically-reduced (no substrate added) as well as in the dithionite- or succinate-reduced minus O2-oxidized difference spectra. The dithionite-reduced plus CO minus reduced difference spectra exhibited cytochromes a3 and o monoxide binding pigments.
这些杆菌是从犰狳身上采集的肉芽肿中分离出来的。采用分光光度法对全细胞悬液以及无细胞提取物中的细胞色素系统进行了检测。完整细胞含有a+b3、b和c型细胞色素，发现它们主要以还原形式存在。麻风分枝杆菌无细胞提取物中的细胞色素系统呈氧化形式，但含有与完整细菌相同类型的细胞色素。在厌氧还原（未添加底物）以及连二亚硫酸盐或琥珀酸盐还原减去O2氧化差光谱中，细胞色素的存在很容易检测到。连二亚硫酸盐还原加CO减去还原差光谱显示出细胞色素a3和一氧化碳结合色素。
