Ishaque M, Kato L, Skinsnes O K
Int J Lepr Other Mycobact Dis. 1977 Apr-Jun;45(2):114-9.
The bacilli were isolated from an armadillo (Dasypus novemcinctus, L.) and cytochrome systems as well as oxidation of succinate and NADH by M. leprae were studied. Cell-free extracts of M. leprae contained cytochromes of the a + a3, b, c and o type. Whole cell suspensions catalyzed the oxidation of succinate. The process was unaffected by rotenone but was markedly inhibited by thenoyltrifluoroacetone, antimycin A and cyanide. Cell-free preparations of M. leprae also oxidized NADH with oxygen as the terminal electron acceptor. Although NADH oxidation was completely inhibited by rotenone, the process was inhibited to only 50% by 5 millimols cyanide. The results indicated that complete respiratory system is present in M. leprae isolated from leprous tissues of an armadillo. The effect of inhibitors on succinate and NADH oxidations showed that the respiration in host-grown M. leprae is mediated through the cytochrome system with oxygen as the final electron acceptor.
从一只犰狳（九带犰狳，L.）中分离出杆菌，并对麻风分枝杆菌的细胞色素系统以及琥珀酸和NADH的氧化进行了研究。麻风分枝杆菌的无细胞提取物含有a + a3、b、c和o型细胞色素。全细胞悬浮液催化琥珀酸的氧化。该过程不受鱼藤酮影响，但明显受到噻吩甲酰三氟丙酮、抗霉素A和氰化物的抑制。麻风分枝杆菌的无细胞制剂也以氧气作为末端电子受体氧化NADH。虽然NADH氧化被鱼藤酮完全抑制，但该过程仅被5毫摩尔氰化物抑制50%。结果表明，从犰狳麻风组织中分离出的麻风分枝杆菌存在完整的呼吸系统。抑制剂对琥珀酸和NADH氧化的影响表明，宿主生长的麻风分枝杆菌中的呼吸作用是通过细胞色素系统介导的，以氧气作为最终电子受体。
