Hrabetová S, Nicholson C
Department of Physiology and Neuroscience, New York University School of Medicine, New York 10016, USA.
J Cereb Blood Flow Metab. 2000 Sep;20(9):1306-10. doi: 10.1097/00004647-200009000-00005.
An unexpected decrease of extracellular space (ECS) tortuosity was recently reported in thick (1,000 microm) ischemic slices using radiotracers. The current study shows that the tortuosity in thick slices from rat neocortex can increase or decrease depending on experimental conditions, whereas ECS volume fraction remains diminished to approximately 10%. Using diffusion of tetramethylammonium, it was found that tortuosity rose from a normoxic value of 1.66 to 1.99 in thick slices. However, tortuosity dropped to 1.54 when dextran (70,000 molecular weight) was added to the bathing medium. The current results show that dextran enhances diffusion in thick ischemic slices without increasing the size of the ECS.
最近有报道称，使用放射性示踪剂在厚（1000微米）的缺血切片中细胞外间隙（ECS）曲折度意外降低。当前研究表明，大鼠新皮质厚切片中的曲折度会根据实验条件而增加或降低，而ECS体积分数仍降至约10%。通过四甲基铵的扩散发现，厚切片中的曲折度从常氧值1.66升至1.99。然而，当向浴液中添加右旋糖酐（分子量70000）时，曲折度降至1.54。当前结果表明，右旋糖酐可增强厚缺血切片中的扩散，而不会增加ECS的大小。
