通过冷冻电子显微镜确定大分子结构。
Macromolecular structure determination by cryo-electron microscopy.
作者信息
Saibil H R
机构信息
Department of Crystallography, Birkbeck College, Malet Street, London WC1E 7HX, England.
出版信息
Acta Crystallogr D Biol Crystallogr. 2000 Oct;56(Pt 10):1215-22. doi: 10.1107/s0907444900010787.
PMID:10998617
Abstract
Recent advances in transmission electron microscopy (EM) hardware, low-temperature methods and image-processing software have made cryo-EM an important complement to X-ray crystallography and NMR for macromolecular structure determination, particularly of large assemblies. This review provides a summary of the main advances and a survey of the capabilities of this approach.
摘要
透射电子显微镜（EM）硬件、低温方法和图像处理软件的最新进展，使冷冻电镜成为X射线晶体学和核磁共振用于大分子结构测定，特别是大型组装体结构测定的重要补充。本文综述了该方法的主要进展，并对其能力进行了概述。
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