Kawamura N, Matsumoto K, Wada A, Goto N
Department of Neurosurgery, Showa University School of Medicine, Tokyo, Japan.
Okajimas Folia Anat Jpn. 2000 Aug;77(2-3):59-62. doi: 10.2535/ofaj1936.77.2-3_59.
Unmyelinated nerve fibers of the oculomotor nerve have occasionally been observed in experimental animals with the use of electron microscopes, but no details concerning normal oculomotor nerves in humans have been published. We measured and analyzed unmyelinated nerve fibers in the human oculomotor nerve with an image analyzer and a computer, using a new staining method, the Luxol fast blue-periodic acid-Schiff-hematoxylin (LPH) discriminative staining method which is the only one suitable for morphometric research on nervous tissues. We studied the numbers and transverse areas of unmyelinated fibers of the oculomotor nerve in 20 cadavers. The number of unmyelinated axons did not change with age, but the mean transverse area decreased with age. These fibers were distributed diffusely in the transverse area of the oculomotor nerve, not localized in any part of the nerve. These findings may be important for the analysis of clinical and neurological signs in relation to aging and ophthalmologic functions.
在实验动物中，曾偶尔使用电子显微镜观察到动眼神经的无髓神经纤维，但尚未发表有关人类正常动眼神经的详细信息。我们使用一种新的染色方法，即Luxol固蓝-过碘酸-希夫-苏木精（LPH）鉴别染色法（这是唯一适用于神经组织形态计量学研究的方法），通过图像分析仪和计算机对人类动眼神经中的无髓神经纤维进行了测量和分析。我们研究了20具尸体动眼神经无髓纤维的数量和横截面积。无髓轴突的数量不随年龄变化，但平均横截面积随年龄减小。这些纤维在动眼神经的横截面积内呈弥漫性分布，不在神经的任何特定部位聚集。这些发现可能对分析与衰老和眼科功能相关的临床和神经学体征具有重要意义。
